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. 2015 Oct 22;44(2):811–823. doi: 10.1093/nar/gkv1074

Figure 2.

Figure 2.

Star-PAP and PAPα compete with each other for CPSF-160 binding. (A) GST-pulldown using GST-Star-PAP or -PAPα to pulldown various CPSF subunits from HEK 293 cell lysates. (B) Quantifications of gels from C–E, and plot of relative bound CPSF-160 fraction on addition of increasing His-Star-PAP or -PAPα to compete the binding of CPSF with other PAP. (C) Control GST-pulldown experiment of CPSF-160 by GST-PAPα with no competitor PAP addition. (D) GST-pulldown of CPSF-160 and hFIP1 by GST-PAPα in the presence of increasing additions of (0, 12.5 nM, 25 nM, 50 nM, 100 nM, 200 nM) His-Star-PAP. (E) GST-pulldown of CPSF-160 and hFIP1 by GST-Star-PAP in the presence of increasing additions of (0, 12.5 nM, 25 nM, 50 nM, 100 nM, 200 nM) His-PAPα. (F–G) GST-pulldown experiment with control GST- from HEK 293 cell lysates in the presence of increasing His-Star-PAP and -PAPα additions.