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. 2015 Oct 20;44(2):669–682. doi: 10.1093/nar/gkv1080

Figure 2.

Figure 2.

The Slx4-Rtt107 complex modulates Rad9 binding to one irreparable DSB. (A) ChIP-seq analysis of Rad9 following induction of a DSB on chromosome III. Rad9 was subjected to chromosome immunoprecipitation at the indicated times after induction of HO endonuclease, in wild type and slx4Δ strains. The enrichment scores (the log2 ratio of immunoprecipitate : input) across 100 kb flanking the HO cut site on chromosome III are plotted. (B–E) ChIP-qPCR analysis showing DSB-induced binding of Rad9, Slx4 or the Slx4-S486A variant at 5 kb from the HO site at the indicated times. All the experiments were performed in nocodazole-blocked cells of the indicated JKM179 derivative strains. In (B), (D) and (E), plotted values are the mean ± SEM of three independent experiments while in (C) two experiments were analyzed. Where indicated, significance was determined by single-tailed Student's t test (*for P < 0.05 and ** for P < 0.01).