Figure 3.

The Slx4-Rtt107 complex modulates long-range DSB resection. (A) Schematic illustration of MAT locus in JKM179 strains showing positions of HO-cut site, SspI restriction sites and the RNA probe used. (B) ssDNA visualization using RNA probe as described in (A) in indicated mutants after inducing HO in nocodazole arrested cells. (C) Resection products analysis by densitometry. Plotted values are the mean values ± SEM from two independent experiments performed as in (B). (D) Schematic illustration of DSB resection at the indicated RsaI sites, 5 kb and 10 kb distal from the irreparable HO cut at the MAT locus on chromosome III. (E–G) DSB resection analysis by qPCR in nocodazole-arrested JKM179 derivative strains. Plotted values are the mean of at least two independent experiments ± SEM. Where indicated, significance was determined by single-tailed Student's t test (*for P < 0.05, ** for P < 0.01 and *** for P < 0.001).