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. 2015 Oct 20;44(2):669–682. doi: 10.1093/nar/gkv1080

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Deletion of SLX4 affects interchromosomal recombination in sae2Δ cells. (A) Schematic illustration of MATa-inc locus in Chromosome III and the additional MATa locus in Chromosome V in tGI354 strain, showing positions of HO-cut site, EcoR1 restriction sites and the probe used to test the interchromosomal recombination. (B) Viability of the tGI354 derivatives after the induction of the HO-cut. (C) Southern blotting analysis of the interchromosomal recombination using the probe as described in (A), in indicated tGI354 derivatives after inducing HO in nocodazole-arrested cells. The intensity of each band was normalized respect to unprocessed IPL1 locus (*). GC is for Gene Conversion. Western blot analysis shows Rad53 phosphorylation of the same experiment. (D) Percentage of crossovers and non-crossovers among all cells in the interchromosomal recombination assay described in (C). (E) Viability of the tGI354 derivatives after the induction of the HO-cut.