Figure 2.

In vitro translation of ErmCL mRNAs carrying modified nucleotides at defined codon positions. (A) SDS-PAGE analysis of in vitro translated mRNA constructs, carrying the depicted modifications at the indicated positions (bold and underlined); mRNAs containing a codon, harboring three deoxy riboses at codon position 15, serve as controls (deoxy) (B) Quantification of at least three independent experiments of in vitro translated mRNAs harboring modified nucleotides at the first (white), second (gray) and third codon position (dark gray), respectively, employing the whole extract system or (C) the PURExpress system. The product yield of the translation of the unmodified mRNA (wt) was set to one. Values are depicted as mean ± SEM.