Table 1. Melting temperatures of the full length and truncated oligonucleotides.
Oligonucleotide | T1/2 (°C) | |
---|---|---|
Low concentration | High concentration | |
32R-3n | 48 | 52 |
5q | 36 | 55 |
3q | 42 (33) | 47 |
T11 | 39 | 40 |
*Concentration of oligonucleotides was in the range between 5 and 10 μM for the low concentration experiment (measured at 295 nm) and between 0.5 and 1.0 mM for the high concentration experiment (measured at 300–305 nm). The temperature was increased/decreased from 10 to 80°C with the rate of 0.5°C/min.