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. 2015 Dec 23;44(2):545–557. doi: 10.1093/nar/gkv1488

Figure 4.

Figure 4.

Distinguishing between kinetic and thermodynamic folded structures of p-shRNA. (A) Schematic and native PAGE gel (15% TBE) for T1 digest of p-shRNA derived from template 7 (one C in one loop). The gel shows prominent bands corresponding to knicked, intact p-shRNA (>500 bp) and a single hairpin unit (∼30 bp). (B) Schematic and native PAGE gel (15% TBE) for T1 digest of p-shRNA derived from template 6 (one C in both loops). The gel shows a single prominent band at ∼30 bp following the T1 digest, corresponding to a knicked single hairpin unit of p-shRNA. Ladders = dsRNA ladder (NEB).