Figure 8.

Imaging cellular RNA transcription using AMUTP 4 under copper-free SPAAC reaction conditions. HeLa cells were transfected with 1 mM of AMUTP 4 for 1 h. The cells were fixed and stained by SPAAC reaction using Cy3-cyclooctyne substrate 14 (red). In a parallel experiment, cells transfected with AMUTP were stained by first SPAAC reaction using 14 (red) followed by CuAAC reaction using Alexa488-alkyne 16 (green). Bottom panel is a merge of all three channels (blue, red and green). Boxed cell has been magnified.