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. 2015 Sep 22;44(2):608–620. doi: 10.1093/nar/gkv958

Figure 1.

Figure 1.

Characterization of suvh1 mutants. (A) The suvh1–1 mutation led to decreased expression of the luciferase gene (LUC) in the YJ background. (Left panel) LUC luminescence of 8-day-old YJ, YJ suvh1–1, YJ suvh1–2 and YJ SUVH1 seedlings grown on MS media. (Right panel) RT-qPCR revealed decreased LUC transcript levels in the suvh1 mutants in the YJ background. ‘YJ SUVH1’ indicates the YJ SUVH1p:SUVH1–3XFLAG suvh1–1 line. In YJ SUVH1, the phenotype of the YJ suvh1–1 mutant was rescued by the transgene containing a wild-type SUVH1 genomic region and a 3XFLAG tag at the C-terminus of SUVH1. (B) A diagram of the SUVH1 protein and the substitution caused by the suvh1–1 mutation. The SUVH1 protein contains an SRA domain, a Pre-SET domain and a SET domain. The G-to-E substitution caused by suvh1–1 occurs in the SET domain. (C) The suvh1–1 mutation led to decreased LUC expression in the LUCH background. (Left panel) LUC luminescence of 8-day-old LUCH and LUCH suvh1–1 seedlings grown on half-strength MS media. (Right panel) RT-qPCR showed decreased LUC expression in the suvh1–1 mutant in the LUCH background. (D–E) The effects of suvh1–1 on LUC expression were suppressed by 5-Aza-2′-deoxycidine treatment in both the YJ (D) and LUCH (E) backgrounds. (Left panels) LUC luminescence of seedlings grown on half-strength MS media containing 7 μg/ml 5-Aza-2′-deoxycytidine for 14 days for YJ and YJ suvh1–1 (D) and LUCH and LUCH suvh1–1 (E). (Right panels) RT-qPCR showed rescued LUC transcript levels in the treated seedlings. Error bars in (A–E) represent standard deviation from three biological replicates.