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. 2015 Sep 22;44(2):608–620. doi: 10.1093/nar/gkv958

Figure 3.

Figure 3.

Analysis of histone methylation marks and expression of known H3K4 methyltransferase genes in suvh1–1. (A and B) ChIP-qPCR was performed to measure H3K9me2 (A) and H3K4me3 (B) levels in YJ and YJ suvh1–1. The UBQ5 gene was included as a control. No changes in H3K9me2 levels were observed at the transgene in the two genotypes. Reduced H3K4me3 levels were observed in YJ suvh1–1 at the LUC coding region but not at the d35S promoter. * Significant difference with a P-value <0.05. ‘–’ represents the sample without antibody; ‘+’ represents the sample with H3K4me3 or H3K9me2 antibodies added. Error bars were calculated from three technical replicates. Results were confirmed by three biological replicates. (C) The expression of known genes encoding H3K4 methyltransferases was determined through RT-qPCR. Error bars were calculated from three biological replicates. UBQ5 was used as an internal control.