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. 2015 Sep 22;44(2):608–620. doi: 10.1093/nar/gkv958

Figure 5.

Figure 5.

Characterizations of SUVH1-targeted loci in nrpe1 and ros1 mutant backgrounds. (A) The expression of four SUVH1-targeted endogenous loci was detected by RT-qPCR in the nrpe1 mutant background. Error bars representing standard deviations were calculated from three biological replicates. * Significant difference with a P-value <0.05. (B) The DNA methylation levels at the promoter regions of SUVH1-targeted endogenous loci were determined through McrBC-qPCR. Locus 1P represents the promoter of locus 1; the same terminology applies to the other loci. Three biological replicates gave similar results, and those from one biological replicate are shown here. (C) Transcript levels of LUC and the four SUVH1-targeted endogenous loci as determined by RT-qPCR. Error bars representing standard deviations were calculated from three biological replicates. * Significant difference with a P-value <0.05.