Figure 2.

Developmental- and tissue-specific gene expression from mat-ICRs associated with bivalent chromatin. Microfluidic-based RT-qPCR analysis of the transcripts initiating from the eight maternal ICRs that are marked by bivalent chromatin in ES cells. Results are presented as the fold enrichment of the mean expression level detected in all tissues, following normalization to the geometrical mean of the expression of the three housekeeping genes Arbp, Gapdh and Tbp. Data were obtained from two independent experiments, each analyzed in duplicate. * Grb10 pat-isoform.