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. 2015 Sep 22;44(2):621–635. doi: 10.1093/nar/gkv960

Figure 6.

Figure 6.

Absence of H3K27me3 affects ICR-associated transcript expression in a locus- and tissue-specific manner. (A and B) Transcription from mat-ICRs associated with bivalent chromatin is mildly affected and only in a subset of loci in Eed−/− ES cells (A) and Ezh2 -/- iMEF cells (B). The results of RT-qPCR analyses are presented as the fold enrichment of the expression level obtained in Eed+/+ ES and Ezh2 +/+ iMEF cells, respectively. Data are from four independent experiments, each analyzed in duplicate. (C) Transcription from maternal ICRs associated with bivalent chromatin in Ezh2−/− somatic cells. Publicly available RNA-seq data were used to produce scatter plots to correlate genome-wide the normalized expression between heterozygotes controls (Het Ezh2 fl) and Ezh2 mutant (Ko Ezh2 fl) E12.5 cardiomyocytes, resting (CD62Lhi) and activated (CD62Llo) spleen T regulatory cells (Tregs). Transcripts initiating from the ICRs are shown by red dots. Peg10 expression is partly affected in Ezh2−/− cardiomocytes, but not in Ezh2−/− Tregs cells compared to controls (Ezh2+/- cells).