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. 2014 Aug 25;63(2):242–256. doi: 10.1002/glia.22748

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© 2014 The Authors. Glia Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Enhanced expression of fibronectin alternatively spliced domains EIIIA and EIIIB mRNA after lysolecithin‐induced demyelination of the mouse spinal cord ventral funiculus. A–C: Real‐time PCR on spinal cord RNA from control mice (unlesioned), at 5 days (5 DPL) or 14 days (14 DPL) post lysolecithin‐induced demyelination. Note the increase in fibronectin EIIIA and EIIIB mRNA at 5 DPL. Gene expression levels were analyzed using the 2^−ΔΔct method (Livak, 2001), with relative expression to HPRT1 or GAPDH. Similar results were obtained for both housekeeping genes; graphs shown the relative expression to GAPDH. Statistical analysis was performed using the Kruskal‐Wallis Test, followed by Dunn's Multiple Comparison Test (* P < 0.05; ** < P 0.01).