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HPLC–MS analysis of in vitro assays with DstH. A) LC–ESI‐MS total ion current traces of DstH‐catalyzed conversion of primycin A1 (2) into the amino form. (B) LC–ESI‐MS total ion current traces of DstH‐catalyzed conversion of kanchanamycin C (3) into the amino form. For the assays shown, Co2+ was the metal ion present. For assays with Ni2+, Zn2+, Mg2+, and Mn2+ as the activating metal ion, see Figures S12, S13.
