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Relative expression level of genes related to the low‐Pi response. Five days after germination Arabidopsis wild‐type (WT) and phr1‐3;phl1‐2 double mutant seedlings were transferred on the indicated media (−phosphate (Pi) = 0 μM Pi; +Pi = 75 μM Pi; + Phostin (PSN)11 = 25 μM PSN11; + dimethyl sulfoxide (DMSO) = 0.1% DMSO) for 4 d before root RNA extraction for quantitative real‐time reverse transcription‐PCR analysis. Error bars, averages ± SE from three independent experiments.
