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Time‐course describing the formation of a Gastruloid over time. Aggregates of small numbers of mESCs plated in low‐adhesion plates will display many of the characteristics of early embryo development such as polarization in gene expression and axial elongation 73, 74. Shown in this figure is the development of a single Gastruloid exposed to a pulse of signalling between 48 and 72 hours. Observe the gradual elongation from one region.
