Table 3.
Fluorimetry assays with 4MU glycosides.a
| 4MU-glycoside/enzyme | Amount of 4MU contaminant (mol %) | 4MU-O−/4MU-glycoside emission ratio | Analytical range | Enzyme activity, μmol · h−1 · L−1 | Percent substrate converted to product |
|---|---|---|---|---|---|
| 4MU-α-L-iduronide/IDUA | 0.067 | 2744 | 16.3 (CDC High) 12.3 (PE High) |
12.5 (CDC High) 9.6 (PE High) |
2.1 (CDC High) 1.6 (PE High) |
| 4MU-α-D-glucose/acid α-glucosidase |
0.023 | 2245 | 16.6 (CDC High) 12.3 (PE High) |
17.5 (CDC High) 10.1 (PE High) |
2.9 (CDC High) 1.7 (PE High) |
| 4MU-α-L-iduronide-2-sulfate/I2S 4MU-sulfate/ARSB |
0.025 0.032 |
2520 58 700 |
11.4 (PE High) 34.0 (PE High) |
8.7 (PE High) 5.0 (PE High) |
1.4 (PE High) 0.16 (PE High) |
| 4MU-β-N-GalNAc-4-sulfate/ARSB | 0.010 | 5390 | 7.8 (PE High) | 5.94 (PE High) | 0.33 (PE High) |
| 4MU-β-N-GalNAc-6-sulfate/GALNS | 0.016 | 4120 | 6.5 (PE High) | 9.14 (PE High) | 0.51 (PE High) |
a
Values calculated as described in the online Data Supplement. PE, PerkinElmer.