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Immunostaining of the iPS cells for several pluripotent cell markers. The mRNA-iPSCs generated and cultured for 15 passages in our xeno-free/feeder-free hPSC system positively immunostained for the pluripotent cell markers Oct4, Sox2, SSEA4, Tra1-60, and Tra1-81. In addition, the colonies were stained with the commonly used undifferentiated cell marker alkaline phosphatase (top right panel).
