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. 2016 Feb 2;11(2):e0147978. doi: 10.1371/journal.pone.0147978

Fig 3. EOC-CM reduced GFAP and VEGF expressions and prevented glucose-induced ROS production by downregulating NOX4 expression in rMC-1 under diabetic milieu.

Fig 3

(a) Representative photomicrograph of GFAP and VEGF immunofluorescence in rMC-1 cultured for 24 hours in high glucose (HG) in presence or not of EOC-CM; magnification: X630. (b) Representative Western blots for NOX4 in rMC-1.Equal loading and transfer were ascertained by reprobing the membranes for ß-actin. (c) The quantification of total intracellular reactive oxygen species (ROS) levels in rMC-1. Values were corrected by the number of cells at the end of each treatment.