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. 2016 Feb 2;11(2):e0147978. doi: 10.1371/journal.pone.0147978

Fig 7. EOC or EOC-CM intravenous injections treatments improve retinal Sirt1 pathway in db/db mice.

Fig 7

(a) A representative photomicrograph of immunolocalization of Sirt-1 in retinal tissue from mice treated with EOC. (b) Western blot for NOX4, Sirt1 and acetyl-Lys310-p65 subunit-NFkBexpression in mice retinal tissue lysate. Equal loading and transfer were ascertained by reprobing the membranes for ß-actin. (b-e) The arbitrary unit of densitometry was transformed to folders of increment in relation to the media of db/m in each experiment to compare independent experiments. (f) A representative photomicrograph of immunolocalization of Sirt1 in retinal tissue from mice treated with EOC-conditioned medium (EOC-CM).