Skip to main content
. 2016 Feb 2;12(2):e1004693. doi: 10.1371/journal.pcbi.1004693

Fig 5. Detection of GS by dot-blot analysis in human embryonic kidney cells.

Fig 5

Glutamine synthetase-YFP-transfected human embryonic kidney cells (HEK293) were either left untreated or treated with the GS-inhibitor L-methionine-S-sulfoximine (MSO) for 2 h. Equal amounts of (A) native or (B) heat- and detergent-denaturated protein were spotted onto a nitrocellulose membrane, and GS was detected by dot-blot analysis followed by densitometric quantification of anti-GS immunoreactivity. Anti-GS immunoreactivity in MSO-treated cells is given relative to the respective untreated control. * Statisticially significantly different compared to untreated controls (p < 0.05). n.s.: not statistically significantly different.