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. 2016 Feb 2;10(2):e0004420. doi: 10.1371/journal.pntd.0004420

Fig 5. Immunofluorescence and ELISA revealed that Nb474 targets a protein in the T. congolense BSF.

Fig 5

(A) Preliminary analysis of Alexa-labeled Nb474 binding to fixed and permeabilized T. congolense BSF parasites reveals green intra-cytoplasmic structures green (panels 3 and 4). (B) Co-localization of Alexa labeled Nb474 and anti-T. brucei aldolase MAb (Anti-TbALD) performed on T. congolense BSF shows the binding of the Nb matched with the Anti-TbALD. (C) The Nb474-ELISA specifically recognizes T. congolense BSF soluble proteome (s.p.), which is an indication of target uniqueness to the BSF.