Table 2.
Titration of anti-FRα MAbs LK26 and 26B3 to cell lines by flow cytometry.
| CELL LINE | ANTIBODY | (µg/mL) | LK26 BINDING | 26B3 BINDING | ||||
|---|---|---|---|---|---|---|---|---|
| MFI1 | S/N RATIO2 | %FRA+ CELLS | MFI1 | S/N RATIO2 | %FRA+ CELLS | |||
| SK-OV-3 | Anti-FRA | 10 | 16,200 | 73 | 100 | 15,500 | 70 | 100 |
| 1 | 11,600 | 52 | 100 | 14,600 | 65 | 100 | ||
| 0.1 | 2,857 | 13 | 99 | 3,553 | 16 | 100 | ||
| Mouse IgG | 10 | 223 | 1 | 25 | 223 | 1 | 25 | |
| HCT-116 | Anti-FRA | 10 | 1,869 | 16 | 98 | 1,317 | 11 | 97 |
| 1 | 1,317 | 11 | 97 | 1,401 | 12 | 97 | ||
| 0.1 | 346 | 3 | 66 | 502 | 4 | 82 | ||
| Mouse IgG | 10 | 118 | 1 | 3 | 118 | 1 | 3 | |
Notes:
1
MFI, mean fluorescence index assessed using flow cytometry.
2
S/N, signal-to-noise ratio is the MFI from anti-FRα antibody/MFI of mouse IgG (control nonspecific mouse antibody) at 10 µg/mL.