Table 3.
Comparison of primary anti-FRα mabs on HCT-116 cells using either FITC or Dylight-649 fluorophores.
| ANTIBODY | (µg/mL) | FITC | DYLIGHT 649 | ||
|---|---|---|---|---|---|
| MFI1 | S/N RATIO2 | MFI1 | S/N RATIO2 | ||
| Clone LK26 | 10 | 342 | 5 | 1,869 | 16 |
| 1 | 276 | 4 | 1,317 | 11 | |
| 0.1 | 122 | 2 | 346 | 3 | |
| Clone 26B3 | 10 | 305 | 4 | 1,317 | 11 |
| 1 | 304 | 4 | 1,401 | 12 | |
| 0.1 | 157 | 2 | 502 | 4 | |
| Mouse IgG | 10 | 71 | 1 | 118 | 1 |
Notes:
1
MFI, mean fluorescence index assessed using Flow Cytometry.
2
S/N, signal-to-noise ratio is the MFI from anti-FRα antibody/MFI of mouse IgG (control nonspecific mouse antibody) at 10 µg/mL.