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Intracellular Ca2+ chelation inhibits zymosan uptake in a Syt VII-dependent manner by MØs, but not DCs. Fluorescent zymosan uptake was analyzed by FACS in Syt VII+/+ or Syt VII−/− MØs (A) and DCs (B) either with or without pretreatment of 20 μM BAPTA-AM. Zymosan was added at 25 particles/cell for 1 h. The results shown are representative of at least three independent experiments.
