Fig. 4.

PI 3-kinase inhibition reduces zymosan uptake by MØs, but not by DCs. (A) FACS profiles of fluorescent zymosan uptake in MØs and DCs left untreated or pretreated with wortmannin (WM) at 5, 50, or 100 nM for 30 min. Zymosan was added at 25 particles/cell for 1 h. The “no zymosan” population reflects autofluorescence of cells without zymosan. (B) Quantification of percent phagocytosis in WM-treated versus untreated, determined from the geometric mean fluorescence of the FACS profiles shown in A. The data represents the mean +/− SD of triplicate experiments. The results shown are representative of at least three independent experiments.