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. Author manuscript; available in PMC: 2016 Feb 2.
Published in final edited form as: Immunobiology. 2009 Jan 20;214(7):495–505. doi: 10.1016/j.imbio.2008.11.006

Fig. 6.

Fig. 6

Syt VII is up-regulated in mature DCs. Syt VII mRNA transcript levels were measured using real-time qPCR on three independent preparations of CD11c+ purified immature and mature DCs. Values were normalized using β-actin transcript levels. The data shown represents +/− SD of triplicate determinations. Inset: 100 μg of whole cell lysates from CD11c+ purified immature (IM) and mature (M) DCs were run on SDS-PAGE and transferred to membrane. Western blotting using affinity purified polyclonal antibody to the amino-terminus of Syt VII detected a 66 kDa band which corresponds to the Syt VII α-isoform. Anti-β-actin antibodies (Abcam) were used as a loading control.

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