Fig. 7.

MHC-II surface transport is delayed in Syt VII-deficient cells. (A) Immature Syt VII^+/+ or Syt VII^−/− DCs were stimulated with LPS and cluster disruption and assessed for MHC-II and CD86 surface expression by FACS at indicated time points up to 12 h. Top profiles compare MHC-II levels, and bottom profiles compare CD86 levels. Syt VII^+/+ DCs (blue), Syt VII^−/− DCs (red). For clarity, the isotype control antibody pattern is only shown for the 12 h time point (right panels, grey). (B) Quantification of the geometric mean fluorescence of the charts shown in A. The results shown are representative of three independent experiments.