Fig. 4.

Macrophage infiltration in glomerular regions was decreased in diabetic Cx3cr1 KO mice. The recruitment of macrophages was examined by immunohistochemical staining with anti-F4/80 (1:200) in renal tissues (a–d; scale bar, 20 µm): (a) WT non-diabetic mice; (b) Cx3cr1 KO non-diabetic mice; (c) WT diabetic mice; and (d) Cx3cr1 KO diabetic mice. The areas staining positive were quantified using Image J software (20 images per animal) (e). The mRNA expression of (f) F4/80 and (g) Mcp-1 was determined by real-time PCR. Data are shown as mean±SE or images showing immunohistochemical staining representative of that obtained in 8–12 mice. ^*p<0.05 vs control Cx3cr1 WT mice; ^†p<0.05 vs diabetic WT mice. Black bars, control; white bars, diabetes. +/+, WT; −/−, Cx3cr1 KO