Fig. 5.

FKN directly induced ECM synthesis through CX3CR1 in MMCs. The mRNA expression of (a) Tgf-β1 (b) Fn and (c) Col4α1 was determined by real-time PCR. In Cx3cr1 siRNA-transfected MMCs, mRNA expression induced by exposure to FKN, 50 mg/ml, of Tgf-β1 (d), Fn (e) and Col4α1 (f) was measured by real-time PCR. Data are mean±SE of four experiments. ^*p<0.05 vs FKN at 0 ng/ml or negative siRNA; ^†p<0.05 vs conditions of FKN and negative siRNA; siScr, negative siRNA; siCx3cr1, Cx3cr1 siRNA