Figure 2. Interactions between SecM-Gly-nascent chain and ribosomal tunnel.

(A) A zoom-in view of the density map in the regions where SecM-Gly-nascent chain interacts with exit tunnel. A transverse-section of the cryo-EM map of the SecM-Gly-RNC, showing P-tRNA and SecM-Gly-nascent chain within the ribosomal exit tunnel is provided in the lower right corner. (B–G) Zoom-in views of the density map in selected regions, highlighting extensive interactions between SecM and ribosomal tunnel components. Map density is shown in mesh, and the atomic model in stick representation. The coloring scheme is the same as in (A). Strong interactions between SecM-nascent-peptide and ribosomal components are indicated by red asterisks. The primary sequences from N-terminal Myc-tag to C-terminal of SecM peptide within the exit tunnel are shown below the panel (F). To illustrate the interaction between the nascent peptide and ribosomal tunnel components, the density map was displayed at relatively lower contour (1.5–3 σ).

DOI: http://dx.doi.org/10.7554/eLife.09684.015

Figure 2—figure supplement 1. Selected regions of interactions between SecM and ribosomal tunnel components in SecM-Gly-RNC.

(A–D) Zoom-in views of the density map in selected regions, highlighting extensive interactions between SecM and ribosomal tunnel components. The coloring scheme is the same as in Figure 2. The interactions between SecM-nascent-peptide and ribosomal components are indicated by red asterisks. The primary sequences from N-terminal Myc-tag to C-terminus of the SecM peptide are shown below the panel (D). To illustrate the interaction between the nascent peptide and ribosomal tunnel components, the density map was displayed at relatively lower contour levels (1.5–3σ).

DOI: http://dx.doi.org/10.7554/eLife.09684.016