Figure 3.

Mouse ES cells with BRCA1 deficient in DBR are sensitive to a PARP inhibitor and HU, but maintain similar ability for HR repair as WT cells. (A) Survival MTT assay using BRCA1-WT cells and BRCA1-ΔDBR cells treated with a PARP inhibitor for 3 days. *P < 0.05, N = 3 (ΔDBR vs WT) (B) Graphical representation of HR assay. (C) HR ability of BRCA1-WT, BRCA1-ΔDBR and BRCA1-Δ11. (MT vs Δ11, *P < 0.01, N = 3); (WT vs Δ11, **P < 0.01, N = 3) (D) Survival MTT assay of BRCA1-WT, BRCA1-ΔDBR and BRCA1-Δ11 after HU for 3 days. *P < 0.05, N = 3 (ΔDBR vs WT)