Fig 2.

NPM1-mA expression promoted adhesion, migration and invasion of THP-1 cells. (A) A cell adhesion assay was performed to test the adhesion ability of the cells. The cells were counted with an MTT assay. The quantified cellular adhesion results were normalized to control cells and are represented as percentages. The relative ratio of the NPM1-mA group was significantly increased compared with the three control groups (p<0.01). (B) Transwell chambers inserted in 24-well tissue culture plates were used for detecting cell migration. The cell numbers of the NPM1-mA group were notably higher than the numbers observed in the NPM1-wt, pEGFPC1 and untreated groups (p<0.01). (C) Transwell chambers with a Matrigel coating were used to test the invasion ability of the cells. Cells that had invaded through the transwell membrane were quantified by counting. The number of invasive cells in the NPM1-mA group was significantly higher than that of the three control groups (p<0.01). Three independent experiments were performed. *p<0.05 vs. control, **p<0.01 vs. controls.