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. 2016 Jan 1;12(2):144–155. doi: 10.7150/ijbs.13382

Fig 5.

Fig 5

The ERK inhibitor, PD95089, affected the expression and activity of MMP-2/9 in THP-1 cells with NPM1-mA overexpression. (A-B) The MMP-2/9 mRNA levels were detected with real time qRT-PCR in the three groups. Decreased MMP-2/9 mRNA was observed in the PD98059 group (MMP-2, p<0.05; MMP-9, p<0.01). (C) MMP-2/9 protein levels were detected by western blotting in the three groups. All the proteins were quantified using image software and normalized against β-actin. A decrease in the MMP-2/9 protein levels was observed in the PD98059 group. (D) The MMP-2/9 protein activity was tested with a gelatin zymography assay in the three groups. The MMP-9 activity was significantly decreased in the PD98059 group compared with the other control groups (p<0.01); however, MMP-2 was not significantly different amongst the three groups (p>0.05). Data are expressed as the means ± SEM of at least 3 independent experiments. *p<0.05 vs. control, **p<0.01 vs. control.