Fig 8.

Binding assay of mutations at the 56th residue in the amyloidogenic core region of lysozyme and GRP78/BiP. I56A, etc.: the single amino acid substitution mutants made of the wild type. (A) Co-immunoprecipitated fractions (IP:FLAG panels) were analyzed by western blotting with anti-lysozyme antibody or the anti-FLAG antibody (marked as Lysozyme and FLAG, respectively). Lysates before immunoprecipitation were also subjected to western blotting (Lysate panels). See the Fig. 2A legend for other descriptions. (B) Localization of selected lysozyme proteins in the GRP78/BiP-positive regions. Details of these procedures and abbreviations are as described in the Fig. 3C legend.