Figure 2.

Oxidative stress in diabetic OVE-GFP podocytes. A. Increased content of lipid peroxidation product 4HNE in diabetic podocytes. B. Increased sensitivity of isolated OVE-GFP podocytes to H₂O₂. n=4 or 5 per group. In A * indicates P<0.05 between PGFP and OVE-GFP by t-test. In B * indicates that 10 µm H₂O₂ significantly reduced cell viability in OVE-GFP podocytes compared to PGFP podocytes at the same dose or OVE-GFP podocytes at lower levels of H₂O₂, P<0.05 by two way ANOVA. Vertical bars are the SEM.