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. 2016 Jan 1;12(2):235–245. doi: 10.7150/ijbs.13814

Figure 3.

Figure 3

Effects of restimulation of osteoclast precursors with M-CSF or RANKL on MAPK activation. (A and B) Osteoclast precursors were treated with M-CSF (M) at 25 ng/mL (A) or RANKL (R) at 500 ng/mL (B) for 2 hours and then restimulated with M-CSF (25 ng/mL) or RANKL (500 ng/mL) for 5 or 10 min, as indicated. Cell lysates were then subjected to immunoblot analysis of MAPK activation. The blots were also subjected to densitometric analysis for determination of each p-MAPK/MAPK ratio. The quantitative data between groups were analyzed by one-way ANOVA comparison. *P < 0.01.