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Optimization of experimental conditions for DNA detection. The effect of hemin concentration (A), enzymatic reaction time (B) and H2O2 incubation time (C) on the sensing system. The target detection was conducted according to the method described in “Experimental section”. The concentration of several species involved is detailed below: [DHP]= 500 nM; [P2]= 500 nM; [p53]= 150 nM; [H2O2]= 83.33 uM; [ABTS]= 0.75 mM.
