Figure 6.
In vivo synergistic HIFU breast cancer surgery. (a) Digital photos of VX2 tumor loaded rabbits before and day 15 after treatment with different treatments. (b) Typical in vivo B-mode ultrasonic images of rabbit VX2 xenograft tumors after injecting 0.2 mL of 6 mg/mL saline control and HMPBs-DOX/PFH before injection (top) and with the subsequent HIFU exposure at 120 W for 5 s (bottom) via intra-tumor injection. The echogenic changes of tumor before and after the ablation were marked by dotted lines, respectively. (c) Tumor volume change of rabbits (n = 6) bearing VX2 treated with different treatments. The tumor volume of control in HMPBs-DOX/PFH +HIFU group (168.2 ± 7.55 %) was apparently lower than that in other groups (e.g., saline: 1603.313 ± 67.261 %) after 15 days observation. (d) The expression of TUNEL in tumor tissue by immunohistochemical examination. The nucleus appears brown (yellow arrow in Ⅰ) was TUNEL-positive cell, blue was negative (green arrow in Ⅵ). (Ⅰ) HMPBs-DOX/PFH + HIFU; (Ⅱ) HMPBs-DOX/PFH; (Ⅲ) HMPBs-PFH + HIFU; (Ⅳ) HMPBs-PFH; (Ⅴ) DOX; (Ⅵ) saline. (e) The PI of TUNEL in HMPBs-DOX/PFH + HIFU group was higher than that in the other three groups (p<0.05). However, there is no significant difference for HMPBs-DOX/PFH + HIFU group to DOX and HMPBs-DOX/PFH group (p>0.05) (f) The immunohistochemical examination images of PCNA in tumor tissue. The nucleus appears blue (green arrow in Ⅰ) was PCNA-negative cell, brown was positive (yellow arrow in Ⅵ). (Ⅰ) HMPBs-DOX/PFH + HIFU; (Ⅱ) HMPBs-DOX/PFH; (Ⅲ) HMPBs-PFH + HIFU; (Ⅳ) HMPBs-PFH; (Ⅴ) DOX; (Ⅵ) saline. (g) The PI of PCNA in the presence of HMPBs-DOX/PFH + HIFU group was lower than that of other five groups (p<0.05).