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. 2016 Jan 25;7:10433. doi: 10.1038/ncomms10433

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(a) SDS-PAGE analysis of the purified CCR4-NOT complex (left) and the CCR4-NOT complex lacking Mmi1 (Mmi1ΔCCR4-NOT complex; right). (b) In vitro deadenylation assay. CCR4-NOT deadenylation was assessed using polyadenylated RNA substrate (34 nucleotides) and the purified CCR4-NOT complex. Samples were collected at 0, 15, 30 and 60 min. The control reaction had no added protein.