Figure 3.
GABAA δ mRNA expression from vlPAG are lower in female rats. A, The designed GABAA δ primer efficiency was 100% (slope: −3.36, r2 = 0.99) and produced a single product (inset). B, There was a significant interaction between treatment and sex (two-way ANOVA, F(3,17) = 3.38, p = 0.0093) when comparing relative GABAA δ mRNA expression. Naive female rats had lower levels than naive male rats (Tukey's post hoc, *p < 0.05). Although there was a strong trend toward an increase in expression with CFA treatment in female rats and a decrease in expression with CFA treatment in males, neither reached significance (N = 4–6 rats/group).