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. 2016 Jan 25;7:10515. doi: 10.1038/ncomms10515

Figure 4. Lipidome analyses of H. influenzae density gradient purified OMVs and OM.

Figure 4

(a,b) PL (a) and FA (b) compositions of OMV and OM preparations derived from Rd KW20, Rd ΔvacJ, and Rd ΔyrbE were analysed by thin-layer chromatography and by gas liquid chromatography in combination with flame-ionization detection, respectively. Mean percentage values with s.e.m. of total PLs (a) and FAs (b) within a given preparation are shown (n=3 biological replicates). Detected PLs and FAs were: phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), myristic acid (C14:0), myristoleic acid (C14:1), palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and cerotic acid (C26:0). (c,d) Total PE contents (c) and PE species compositions (d) of respective OMV and OM preparations were determined by LC/ESI-MS. Total PE contents (c) are given in x-fold changes normalized to Rd KW20 OMV preparations and PE species compositions (d) are given in percentage of total PE species within a respective preparation. Mean values with s.e.m. are shown (n=3 biological replicates). For PE composition analysis (d), only PE species over 1% (at least in one preparation) are shown. The main FAs of a given PE species are indicated below each species. Additional detected FAs: capric acid (C10:0), lauric acid (C12:0), and lauroleic acid (C12:1). (b,c,d) Significant differences between the data sets are marked by asterisks (P<0.05; one-way ANOVA followed by Sidak's multiple comparison post test).