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. 2016 Jan 21;7(1):e138. doi: 10.1038/ctg.2015.66

Figure 6.

Figure 6

hsp60 gene expression and Hsp60 protein levels in the liver. (a) The bars show the levels of hsp60 gene expression normalized for the reference genes, according to the Livak method (2−ΔΔCT), in the liver of control (Con), ethanol diets, and ethanol and probiotic diet mice. (b) Western blotting results. Ratio Hsp60 levels/β-actin levels as a reflection of Hsp60 increase (mean±s.d.). (c) Representative cropped western blots for Hsp60 in control and ethanol-fed mice. All gels were run under the same experimental conditions and β-actin was used as an internal control. *P<0.01 vs. 4EtOH-P, 8EtOH; #P<0.05 vs. 12EtOH-P; §P<0.05 vs. Con and 4EtOH; ¥ P<0.05 vs. Con, 4EtOH-P. For the meaning of the abbreviations in a and b, indicating each mouse group, see legend for Figure 1 and Table 1. AU, arbitrary unit; Con, control; 4EtOH, ethanol-fed mice for 4 weeks; 8EtOH, ethanol-fed mice for 8 weeks; 12EtOH, ethanol-fed mice for 12 weeks; 4EtOH-P, ethanol and probiotic-fed mice for 4 weeks; 8EtOH-P, ethanol and probiotic-fed mice for 8 weeks; 12EtOH-P, ethanol and probiotic-fed mice for 12 weeks; 8EtOH-4P, ethanol-fed mice for 8 weeks and then given probiotic for 4 weeks; wks, weeks.