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. 2016 Feb 2;11(2):e0147737. doi: 10.1371/journal.pone.0147737

Fig 1. Effect of uric acid (UA) on 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-NBDG) uptake and Akt phosphorylation in H9c2 cardiomyocytes.

Fig 1

(A, B, D, E) Flow cytometry of cells pretreated with UA (0, 5, 10, and 15 mg/dl) or 0, 30 min and 1, 8, 16 and 24 h, then assayed for insulin-stimulated 2-NBDG uptake. (C, F) Western blot analysis of phosphorylated and total Akt levels. *P<0.05 vs. 0, 30 min, and 1 h. #P<0.05 vs. 0, 5, and 10 mg/dl. GAPDH was a normalization control.