Fig 2.

(A-C) Effect of HUA on 2-NBDG uptake in H9c2 cardiomyocytes. Cells were pretreated with HUA, then underwent basal or insulin-stimulated 2-NBDG uptake assay detected by fluorescence microscopy (A) and analyzed by flow cytometry (B). (C) *P<0.01 vs. control, #P<0.01 vs. insulin and HUA. (D-E) Effect of HUA on ROS generation in H9c2 cardiomyocytes. Cells were co-incubated with HUA and stained with DCFH-DA for fluorescence microscopy (D) and analyzed by flow cytometry (E). (F) *P<0.01 vs. control, #P<0.01 vs. HUA and N-acetyline cystein (NAC). (G-I) Effect of HUA on 2-NBDG uptake in H9c2 cardiomyocytes via oxidative stress. Cells were pretreated with HUA with or without NAC, then underwent basal or insulin-stimulated 2-NBDG uptake assay detected by fluorescence microscopy (G) and flow cytometry (H). (I) *P<0.01 vs. NAC, #P<0.01 vs. NAC+HUA. Data are mean±SD from 3 separate experiments.