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. 2016 Feb 3;10:14. doi: 10.3389/fncel.2016.00014

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Copyright © 2016 Martin, Bonner, Elkjær, D’Orsi, Chen, König, Svensson, Deierborg, Pfeiffer, Prehn and Lambertsen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cerebrovascular anatomy. (A) The territory of the middle cerebral artery (MCA) was measured as the distance from the line of anastomoses between the MCA and the anterior cerebral artery (ACA) to the midline at 2, 4 and 6 mm from the frontal pole. The cerebrovascular anatomy was analyzed by visualizing the arteries with cresyl violet solution. We observed no significant difference between BID-KO mice and WT mice at any of these three sites (p = 0.96, n = 10–13 in each group, 2-way ANOVA mean ± SD). (B) The diameters of the posterior communicating arteries (PcomAs) were measured at the widest part and the values were calculated as a percentage of the basilar artery (BA) diameter. We found no significant difference between the two groups of mice (p = 0.11, n = 10–13 in each group, non-paired t-test, mean ± SD). SCA: Superior cerebellar artery.