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. 2016 Feb 3;10:14. doi: 10.3389/fncel.2016.00014

Figure 5.

Figure 5

mRNA expression of downstream regulators of mitochondrial mediated apoptosis. The mRNA level of BAX, BAK, Bcl2 and Bclxl was examined by qPCR on whole brain lysates and compared between BID-KO mice and WT mice exposed to tMCAO or sham surgery. We found no significant difference between BID-KO mice and WT mice for any of the examined mitochondrial apoptosis regulator genes. Overall the direction of expression was however similar for all four examined genes after tMCAO with the highest expression level found in BID-KO mice (BAK: p = 0.34, BAX: p = 0.44, Bcl2: 0.33, Bclxl: p = 0.25, n = 3 in each sham group, n = 5–6 in each tMCAO group, mean ± SD). There was a significantly lower Bcl2 expression level in tMCAO mice compared to sham mice (#p = 0.047), but not for BAX, BAK or Bclxl (p = 0.65, p = 0.42, p = 0.63 respectively, n = 3 in each sham group, n = 5–6 in each tMCAO group, non-paired t-test, mean ± SD).