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. 2016 Feb 3;10:14. doi: 10.3389/fncel.2016.00014

Figure 6.

Figure 6

Microglial/leukocyte response after transient middle cerebral artery occlusion (tMCAO). (A) IBA1 staining of ipsilateral and contralateral hemisphere of one representative BID-KO and WT mouse exposed to tMCAO. We observed no significant difference in the number of IBA1+ cells per field of view between BID-KO mice and WT mice exposed to tMCAO on the contralateral hemisphere or the ipsilateral hemisphere (p = 0.93), however, in the brains of WT mice, the expression was significantly higher in the ipsilateral hemisphere compared to the contralateral hemisphere (*p = 0.017). This difference was not observed in the brains of BID-KO mice. The white arrow marks an IBA1 positive cell. The pictures were taken from normal appearing cortex. Scale bars: 60 μm (n = 5–6 in each group, 2-way ANOVA, mean ± SD). (B) The relative mRNA expression of CD38 was significantly higher in BID-KO mice compared to WT mice exposed to tMCAO 24 h after surgery (*p = 0.05, n = 3 in each sham group, n = 5–6 in each tMCAO group, 2-way ANOVA, mean ± SD). (C) The relative mRNA expression level of CD11b was not quite upregulated 24 h after surgery (p = 0.073), and there was no significant difference between BID-KO and WT sham mice or between BID-KO and WT tMCAO mice (p = 0.87, n = 3 in each sham group, n = 5–6 in each tMCAO group, 2-way ANOVA, mean ± SD).