Figure 4.
Droplet mixer with fluorescence detection. (a) Image of the droplet mixer along with numbered boxes, corresponding to the fluorescence images. (b) Box 1 shows the region where the fluorescein (from left) and KI solution (from top) streams merged. Box 2 shows the region where the aqueous fluorescein/KI solution (from top) meets the organic hexanes (from left) and the droplet is starting to form. Box 3, which has been rescaled to show contrast, shows the fluorescein/KI droplet (moving left to right) at a point equivalent to 52 ms downstream from the aqueous/organic merge point. Box 4, which has been rescaled to show contrast, shows the droplet farther downstream (moving left to right), equivalent to 122 ms from the aqueous/organic merge point. The contrast of each image was adjusted to make important details visible (the maximum intensity decreases from left to right), therefore the apparent brightness in each image does not reflect actual fluorescence intensity. (c) Plot of the average fluorescence intensity (counts) with respect to each of the imaged regions of the mixer.